KO-Validated
Polyclonal Antibody to Integrin Alpha V (ITGaV)

CD51; ITG-AV; MSK8; VNRA; Vitronectin Receptor,Alpha Polypeptide

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 100.00 US$ 232.00 US$ 332.00 US$ 830.00 US$ 3,320.00
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Overview

Properties

Product No.PAB282Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryCD & Adhesion molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB282Hu01-Recombinant Integrin Alpha V (ITGaV)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

IHC Images
AF488 staining on IF;
Sample: A549 cell
Primary Ab: 20µg/ml Rabbit Anti-Human ITGaV Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant ITGaV, Human.
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: ITGaV knockout A549 cell lysate;
Predicted MW: 116kDa
Observed MW: 130kDa
Primary Ab: 4µg/ml Rabbit Anti-Human ITGaV Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ITGaV. It has been selected for its ability to recognize ITGaV in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Vitronectin, Integrin Vitronectin Receptor Other Biochemical Markers to Assess the Development of Liver CirrhosisISSN: 0975 -8542
PET imaging of Ga-NODAGA-RGD, as compared with F-fluorodeoxyglucose, in experimental rodent models of engrafted glioblastomaPubmed:29904818
Mechanically induced formation and maturation of 3D-matrix adhesions (3DMAs) in human mesenchymal stem cellsPubmed: 32818825

KO-Validated Polyclonal Antibody to Integrin Alpha V (ITGaV)