Polyclonal Antibody to Jagged 1 (JAG1) Homo sapiens (Human) Polyclonal antibody

CD339; AGS; AHD; AWS; HJ1; JAGL1; Alagille Syndrome; Jagged 1 Protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB807Hu03-Recombinant Jagged 1 (JAG1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Jagged 1 (JAG1) Packages (Simulation)
  • Polyclonal Antibody to Jagged 1 (JAG1) Packages (Simulation)
  • Polyclonal Antibody to Jagged 1 (JAG1) IHC Images
  • Polyclonal Antibody to Jagged 1 (JAG1) Vector Red staining on IHC-P;
    Samples: Human Colon Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human JAG1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
  • Polyclonal Antibody to Jagged 1 (JAG1) Vector Red staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human JAG1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
  • Polyclonal Antibody to Jagged 1 (JAG1) AF488 staining on IF;
    Sample: Hepa1-6 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human JAG1 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB807Hu03.jpg Figure. Western Blot; Sample: Recombinant JAG1, Human.
  • Polyclonal Antibody to Jagged 1 (JAG1) Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Porcine Liver Tissue; Lane4: Mouse Liver Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against JAG1. It has been selected for its ability to recognize JAG1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Cross-talk between the Toll-like receptor 4 and Notch1 pathways augments the inflammatory response in the interstitial cells of stenotic human aortic valvesPubMed: PMC3506187
  • Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosisPubmed: 24188612
  • Augmented Osteogenic Responses in Human Aortic Valve Cells Exposed to oxLDL and TLR4 Agonist: A Mechanistic Role of Notch1 and NF-κB InteractionPubmed:Pmc4014478
  • Ligation of ICAM-1 on human aortic valve interstitial cells induces the osteogenic response: A critical role of the Notch1-NF-κB pathway in BMP-2 expressionPubmed:25101972
  • Jagged1 Stimulates Bone Resorption during Orthodontic Tooth MovementDn: Jlc
  • MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer
  • Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathyPubmed: 31511328
  • Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria33795655

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