• KO-Validated
Polyclonal Antibody to Keratin 1 (KRT1) Homo sapiens (Human) Polyclonal antibody

CK1; EHK1; K1; KRT1A; KRT1-A; Cytokeratin 1; Epidermolytic Hyperkeratosis; Keratin, type II cytoskeletal 1; 67 kDa cytokeratin; Type-II keratin Kb1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA492Hu01-Recombinant Keratin 1 (KRT1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Keratin 1 (KRT1) Packages (Simulation)
  • Polyclonal Antibody to Keratin 1 (KRT1) Packages (Simulation)
  • Polyclonal Antibody to Keratin 1 (KRT1) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human KRT1 Ant
  • Polyclonal Antibody to Keratin 1 (KRT1) DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Keratin 1 (KRT1) Figure. DAB staining on IHC-P;
    Samples: Human Liver Cancer Tissue.
  • PAA492Hu01.jpg Figure. Western Blot; Sample: Recombinant KRT1, Human.
  • Polyclonal Antibody to Keratin 1 (KRT1) Figure. Western Blot; Sample: Lane1: Human A375 Cells; Lane2: Human H460 Cells; Lane3: Human Hela Cells; Lane4: Mouse Pancreas Tissue.
  • Polyclonal Antibody to Keratin 1 (KRT1) Knockout Varification:
    Lane 1: Wild-type A431 cell lysate;
    Lane 2: KRT1 knockout A431 cell lysate;
    Predicted MW: 66kDa
    Observed MW: 66kDa
    Primary Ab: 3µg/ml Rabbit Anti-Human KRT1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against KRT1. It has been selected for its ability to recognize KRT1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:350-1400
Immunohistochemistry: 5-20µg/mL;1:35-140
Immunocytochemistry: 5-20µg/mL;1:35-140
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

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