Polyclonal Antibody to Lactate Dehydrogenase B (LDHB)

LDH-B; LDH-H; Renal carcinoma antigen NY-REN-46

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 109.00 US$ 253.00 US$ 362.00 US$ 905.00 US$ 3,620.00
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Overview

Properties

Product No.PAB698Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Cardiovascular biology

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB698Ra01-Recombinant Lactate Dehydrogenase B (LDHB)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Rat Heart Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat LDHB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Stomach Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Rat LDHB Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat LDHB Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat LDHB Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Brain Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat LDHB Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat LDHB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Stomach Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat LDHB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Rat LDHB Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant LDHB, Rat.
Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Jurkat cell lysate; Lane4: A431 cell lysate
Primary Ab: 0.01µg/ml Rabbit Anti-Rat LDHB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Mouse Cerebrum lysate; Lane3: Rat Heart lysate;
Primary Ab: 0.01µg/ml Rabbit Anti-Rat LDHB Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against LDHB. It has been selected for its ability to recognize LDHB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

Giveaways

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Citations

Proteomic analysis of effluents from perfused human heart for transplantation: identification of potential biomarkers for ischemic heart damagProteomesci: 14775956
Presence in the Pre-Surgical Fine-Needle Aspiration of Potential Thyroid Biomarkers Previously Identified in the Post-Surgical OnePubMed: PMC3765839
LDHB inhibition induces mitophagy and facilitates the progression of CSFV infectionPubmed: 32924761
Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in micePubmed: 32821439