Polyclonal Antibody to Pancreatic Lipase (PL) Rattus norvegicus (Rat) Polyclonal antibody

PNLIP; PTL; Pancreatic Lipase; Pancreatic triacylglycerol lipase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB453Ra01-Recombinant Pancreatic Lipase (PL)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Pancreatic Lipase (PL) Packages (Simulation)
  • Polyclonal Antibody to Pancreatic Lipase (PL) Packages (Simulation)
  • Polyclonal Antibody to Pancreatic Lipase (PL) DAB staining on IHC-P; Sample: Rat Pancreas Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat PL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pancreatic Lipase (PL) DAB staining on IHC-P;
    Sample: Rat Pancreas Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat PL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB453Ra01.jpg WB Image
  • Polyclonal Antibody to Pancreatic Lipase (PL) Western Blot; Sample: Lane1: Rat Pancreas lysate; Lane2: Mouse Pancreas lysate
    Primary Ab: 0.005µg/ml Rabbit Anti-Rat PL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pancreatic Lipase (PL) Western Blot; Sample: Rat Pancreas lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat PL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PL. It has been selected for its ability to recognize PL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Mechanistic investigations of test?article-induced?pancreatic toxicity at the endocrine-exocrine interface in the rat.Pubmed:24226507
  • Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevationPubmed:27243230
  • Abdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial34024503
  • Associations between Intra-Pancreatic Fat Deposition, Pancreas Size, and Pancreatic Enzymes in Health and after an Attack of Acute Pancreatitis34753126

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