Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) Homo sapiens (Human) Polyclonal antibody

HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB752Hu01-Recombinant Major Histocompatibility Complex Class I E (MHCE)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) Packages (Simulation)
  • Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) Packages (Simulation)
  • Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) DAB staining on IHC-P; Sample: Mouse Uterus Tissue Primary Ab: 10µg/ml Rabbit Anti-Human MHCE Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) DAB staining on IHC-P;
    Sample: Mouse Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human MHCE Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) DAB staining on IHC-P;
    Sample: Mouse Spleen Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human MHCE Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) AF488 staining on IF;
    Sample: Raji cell
    Primary Ab: 20µg/ml Rabbit Anti-Human MHCE Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB752Hu01.jpg Figure. Western Blot; Sample: Recombinant MHCE, Human.
  • Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE) Western Blot; Samples: Lane1: THP1 cell lysate; Lane2: Raji cell lysate; Lane3: Ramos cell lysate; Lane4: HUVEC cell lysate;
    Primary Ab: 0.3µg/ml Rabbit Anti-Human MHCE Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MHCE. It has been selected for its ability to recognize MHCE in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • HLA–E gene polymorphism associated with susceptibility to kawasaki disease and formation of coronary artery aneurysmsAsia: source
  • Soluble-HLA-E: A follow up biomarker in Takayasu arteritis, independent of HLA-E genotypepubmed:28425192
  • Immune signatures of treatment resistant schizophrenia: a FACE-SZ study
  • HLA-E circulating and genetic determinants in schizophrenia and bipolar disorder34642395

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