Polyclonal Antibody to Mannose Binding Lectin (MBL) Mus musculus (Mouse) Polyclonal antibody

MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB480Mu01-Recombinant Mannose Binding Lectin (MBL)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Mannose Binding Lectin (MBL) Packages (Simulation)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) Packages (Simulation)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) DAB staining on IHC-P; Sample: Mouse Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse MBL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) DAB staining on IHC-P;
    Sample: Mouse Liver Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse MBL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB480Mu01.jpg Figure. Western Blot; Sample: Recombinant MBL, Mouse.
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) Western Blot; Sample: Lane1: Mouse Serum; Lane2: Rat Liver lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse MBL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Rat Serum
    Primary Ab: 0.6µg/ml Rabbit Anti-Mouse MBL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MBL. It has been selected for its ability to recognize MBL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. Karger: 000255439
  • Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle GlaucomaIngenta: art00007
  • Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
  • Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathyPubMed: PMC3406374
  • Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GNPubmed: 24115193
  • Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensisPubmed: 32827456
  • Complement activation profile of patients with primary focal segmental glomerulosclerosisPubmed: 32569286
  • The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes34242658

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