Polyclonal Antibody to Mannose Binding Lectin (MBL) Rattus norvegicus (Rat) Polyclonal antibody

MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB480Ra01-Recombinant Mannose Binding Lectin (MBL)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Mannose Binding Lectin (MBL) Packages (Simulation)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) Packages (Simulation)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) DAB staining on IHC-P; Samples: Rat Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat MBL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) DAB staining on IHC-P;
    Sample: Rat Liver Tissue;
    Primary Ab: 10ug/ml Rabbit Anti-Rat MBL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) AF488 staining on IF;
    Sample: Hepa1-6 cell
    Primary Ab: 20µg/ml Rabbit Anti-Rat MBL Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB480Ra01.jpg WB Image
  • Polyclonal Antibody to Mannose Binding Lectin (MBL) Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Liver lysate; Lane3: Mouse Serum
    Primary Ab: 0.1µg/ml Rabbit Anti-Rat MBL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MBL. It has been selected for its ability to recognize MBL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. Karger: 000255439
  • Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle GlaucomaIngenta: art00007
  • Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
  • Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathyPubMed: PMC3406374
  • Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GNPubmed: 24115193
  • Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensisPubmed: 32827456
  • Complement activation profile of patients with primary focal segmental glomerulosclerosisPubmed: 32569286
  • The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes34242658

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