Polyclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Homo sapiens (Human) Polyclonal antibody

ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Packages (Simulation)
  • Polyclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Packages (Simulation)
  • Polyclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) DAB staining on IHC-P; Sample: Rat Colon Tissue Primary Ab: 10µg/ml Rabbit Anti-Human MANF Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) DAB staining on IHC-P;
    Sample: Rat Cerebrum Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human MANF Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) DAB staining on IHC-P;
    Sample: Rat Pancreas Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human MANF Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC300Hu01.jpg Figure. Western Blot; Sample: Recombinant MANF, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MANF. It has been selected for its ability to recognize MANF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
  • Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.pubmed:28216543
  • Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signalingpubmed:28263910
  • Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance10.1507:endocrj.EJ16-0472
  • MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage
  • Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway33856409
  • The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia …34234505

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