Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) Homo sapiens (Human) Polyclonal antibody

MAPK8; JNK1A2; JNK21B1/2; PRKM8; SAPK1; JUN N-Terminal Kinase; Stress-activated protein kinase 1c; Mitogen Activated Protein Kinase 8

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB156Hu01-Recombinant c-Jun N-terminal Kinase 1 (JNK1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.37mg/ml
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  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) Packages (Simulation)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) Packages (Simulation)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human MAPK8 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) DAB staining on IHC-P;
    Samples: Human Spleen Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human MAPK8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) DAB staining on IHC-P;
    Samples: Human Small intestine Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human MAPK8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) DAB staining on IHC-P;
    Samples: Human Cardiac Muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human MAPK8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) DAB staining on IHC-P;
    Sample: Human Small intestine Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human JNK1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human JNK1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) DAB staining on IHC-P;
    Sample: Human Spleen Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human JNK1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) FITC staining on IF;
    Sample: Human Hela cell;
    Primary Ab: 20μg/ml Rabbit Anti-Human JNK1 Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) FITC staining on IF;Sample: Hela cellPrimary Ab: 20µg/ml Rabbit Anti-Human JNK1 AntibodySecond Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb11)
  • PAB156Hu01.jpg Figure. Western Blot; Sample: Recombinant MAPK8, Human.
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: A431 cell lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Human MAPK8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1) Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Bovine Cerebrum lysate; Lane3: Canine Cerebrum lysate; Lane4: Gallus Cerebrum lysate; Lane5: Caprine Cerebrum lysate; Lane6: Cavia Cerebrum lysate; Lane7: Equine Lung lysate
    Primary Ab: 0.5µg/ml Rabbit Anti-Human JNK1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against JNK1. It has been selected for its ability to recognize JNK1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

Citations

  • EGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expressionPubmed: 32703964

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