• KO-Validated
Polyclonal Antibody to SMAD family member 3 (SMAD3) Homo sapiens (Human) Polyclonal antibody

JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC123Hu01-Recombinant SMAD family member 3 (SMAD3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to SMAD family member 3 (SMAD3) Packages (Simulation)
  • Polyclonal Antibody to SMAD family member 3 (SMAD3) Packages (Simulation)
  • Polyclonal Antibody to SMAD family member 3 (SMAD3) IHC Images
  • PAC123Hu01.jpg Figure. Western Blot; Sample: Recombinant Smad3, Human.
  • Polyclonal Antibody to SMAD family member 3 (SMAD3) Western Blot; Sample: Rat Pancreas lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 3 (SMAD3) Knockout Varification:
    Lane 1: Wild-type PC3 cell lysate;
    Lane 2: Smad3 knockout PC3 cell lysate;
    Predicted MW: 26,36,43,48kd
    Observed MW: 43kd
    Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 3 (SMAD3) Western Blot; Sample: Human PC3 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 3 (SMAD3) Western Blot; Sample: Human 293T cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 3 (SMAD3) Western Blot; Sample: Human Jurkat cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SMAD3. It has been selected for its ability to recognize SMAD3 in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model

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