Polyclonal Antibody to Thioredoxin (Trx) Mus musculus (Mouse) Polyclonal antibody

TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA702Mu01-Recombinant Thioredoxin (Trx)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Thioredoxin (Trx) Packages (Simulation)
  • Polyclonal Antibody to Thioredoxin (Trx) Packages (Simulation)
  • Polyclonal Antibody to Thioredoxin (Trx) DAB staining on IHC-P; Sample: Mouse Ovary Tissue Primary Ab: 10µg/ml Rabbit Anti-Mouse Trx Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Thioredoxin (Trx) DAB staining on IHC-P;
    Sample: Mouse Colon Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse Trx Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA702Mu01.jpg Figure 1. Western Blot; Sample: Recombinant Trx, Mouse.
  • Polyclonal Antibody to Thioredoxin (Trx) Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Liver lysate; Lane3: Rat Cerebrum lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Mouse Trx Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against Trx. It has been selected for its ability to recognize Trx in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.1-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis OxfordJournals: source
  • Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosusPubmed:Pmc4061188
  • Alteration in the intrafollicular thiol–redox system in infertile women with endometriosisPubmed:25376627
  • Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stressPubmed:25564095
  • Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung.Pubmed:26797643
  • Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the …Pubmed: 30351992
  • Redox, immune and genetic biomarker system for personalized treatments in colorectal cancerDoi: 10.4251/wjgo.v11.i2.117
  • The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in TriathletesPubmed: 30736479
  • Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitusPubmed: 31351037
  • Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
  • Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ responsePubmed: 33161477
  • Examination of androgenetic alopecia with serum biomarkersPubmed: 32969583
  • Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats34159266
  • Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat34155936

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