Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) Homo sapiens (Human) Polyclonal antibody

NAGase; MEA5; NCOAT; Hexosaminidase C; N-Acetylhexosaminidase; Hyaluronidase; Meningioma-Expressed Antigen 5; Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA069Hu01-Recombinant Meningioma Expressed Antigen 5 (MGEA5)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) Packages (Simulation)
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) Packages (Simulation)
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human NAGase Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human MGEA5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Human NAGase Antibody
    Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) FITC staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human MGEA5 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA069Hu01.jpg Figure. Western Blot; Sample: Recombinant NAGase, Human.
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Hela cell lysate; Lane3: MCF7 cell lysate; Lane4: U87MG cell lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Human IGF1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human NAGase Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Meningioma Expressed Antigen 5 (MGEA5) Western Blot; Sample: Rat Cerebrum lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human NAGase Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MGEA5. It has been selected for its ability to recognize MGEA5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Metal-on-Metal Hip Prostheses and Systemic Health: A Cross-Sectional Association Study 8 Years after ImplantationPubMed: PMC3677913
  • Evaluation of the Process of Recycling and Renal Parenchymal Injury after ESWL with Metabolites Excreted in the UrinePubmed: 23413820
  • The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary StudiesHindawi: 740105
  • Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in RatsPubMed: 25909514
  • Melamine and oxalate coexposure induces early kidney tubular injury through mitochondrial aberrations and oxidative stress34507040
  • Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 146434573096

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