Polyclonal Antibody to NADH Dehydrogenase 5 (ND5)

MT-ND5; MTND5; NAD5; NADH-Ubiquinone Oxidoreductase Chain 5; Mitochondrially Encoded NADH Dehydrogenase 5

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 125.00 US$ 293.00 US$ 418.00 US$ 1,045.00 US$ 4,180.00
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Overview

Properties

Product No.PAS952Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPS952Ra01-Recombinant NADH Dehydrogenase 5 (ND5)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Rat Liver Tissue Primary Ab: 10µg/ml Rabbit Anti-Rat ND5 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant ND5, Rat.
Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Heart lysate; Lane3: Rat Cerebrum lysate
Primary Ab: 5µg/ml Rabbit Anti-Rat ND5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Serum
Primary Ab: 5µg/ml Rabbit Anti-Rat ND5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Serum
Primary Ab: 5µg/ml Rabbit Anti-Rat ND5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ND5. It has been selected for its ability to recognize ND5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

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Citations

In vitro antoxidant eﬀect of curcumin on human sperm quality in leucocytospermiadoi:10.1111
In vitro antioxidant effect of curcumin on human sperm quality in leucocytospermiapubmed:28133770
In vitro antioxidation effect of Quercetin on sperm function from the infertile patients with leukocytospermiaPubmed: 31166052