Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)

DHQU; DIA4; DTD; NMOR1; NMORI; QR1; Azoreductase; DT-diaphorase; Menadione reductase; Phylloquinone reductase; Quinone reductase 1; NAD(P)H:quinone oxidoreductase 1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 116.00 US$ 270.00 US$ 386.00 US$ 965.00 US$ 3,860.00
Quantity

Add to Cart Distributors

Overview

Properties

Product No.PAL969Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPL969Hu01-Recombinant NADH Dehydrogenase, Quinone 1 (NQO1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

Share your citation Upload your experimental result Review Leave a message

Sign into your account

Email *

Password *

Verification code*

Create a new account

Share a new citation as an author

Title *

Journal

Link*

Upload your experimental result

Application*

Experimental result*

Review

Rating

Serial No* Please attach serial No. on instruction manual

Contact us
Please fill in the blank.

Name*

Organization

Address

E-mail address*

Telephone

Inquiry*

Verification code* CheckCode

Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: A549 cell
Primary Ab: 20µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Figure. Western Blot; Sample: Recombinant NQO1, Human.
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human NQO1 Polyclonal Antibody (Catalog PAL969Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against NQO1. It has been selected for its ability to recognize NQO1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Increment services

Citations

Transcription factor Nrf2 protects renal dopamine D1 receptor function during oxidative stress.Pubmed: 23876469
Notoginsenoside R1-mediated neuroprotection involves estrogen receptor-dependent crosstalk between Akt and ERK1/2 pathways: A novel mechanism of Nrf2/ARE signaling activationInformahealthcare: Source
Antioxidant resveratrol restores renal sodium transport regulation in SHRPubMed: 26603454
Genetic association of NAD (P) H Quinone Oxidoreductase (NQO1* 2) polymorphism with NQO1 levels and risk of diabetic nephropathyPubmed:27078674
Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazolePubmed: 30790573
Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug InteractionsPubmed: 32764932
In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanismPubmed:35594934