Polyclonal Antibody to Netrin 1 (Ntn1)
NTN1L; Epididymis tissue protein Li 131P
Overview
Properties
- Product No.PAB827Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryNeuro science
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB827Ra01-Recombinant Netrin 1 (Ntn1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against Ntn1. It has been selected for its ability to recognize Ntn1 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;1:120-500
Immunohistochemistry: 5-20µg/mL;1:12-50
Immunocytochemistry: 5-20µg/mL;1:12-50
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Homo sapiens (Human)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Netrin-1: A Novel Universal Biomarker of Human Kidney InjuryPubMed: 20620466
- Plasma netrin-1 is a diagnostic biomarker of human cancersPubMed: PMC3143477
- Cord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growthInformahealthcare: Source
- Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesityPubmed:24584118
- Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growthPubmed:24716747
- Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injuryPubmed:25154466
- Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC)articles:10.1186
- The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium.pubmed:29156815
- Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in RatsPubmed:29375318
- Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptorPubmed:29662125
- Relationship of serum netrin-1 levels with breast massesDoi: 10.22391/fppc.467700
- Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesityPubmed: 30910812
- Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes MellitusPubmed: 30897795
- Serum netrin-1 as a biomarker for colorectal cancer detectionPubmed: 32474463
- Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos
- Association of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD33853303
- Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture TapePubmed:35670309