Polyclonal Antibody to NADPH Oxidase 4 (NOX4)

KOX; KOX-1; RENOX; Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 100.00 US$ 232.00 US$ 332.00 US$ 830.00 US$ 3,320.00
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Overview

Properties

Product No.PAB924Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Enzyme & Kinase Metabolic pathway Tumor immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB924Hu01-Recombinant NADPH Oxidase 4 (NOX4)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Mouse Kidney Tissue Primary Ab: 10µg/ml Rabbit Anti-Human NOX4 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Vector Red staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 15µg/ml Rabbit Anti-Human NOX4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Figure. Western Blot; Sample: Recombinant NOX4, Human.
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NOX4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NOX4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against NOX4. It has been selected for its ability to recognize NOX4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

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Citations

Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4PubMed: 26522475
Oxidative stress promotes benign prostatic hyperplasiaPubMed: 26417670
Effects of Single and Combined Losartan and Tempol Treatments on Oxidative Stress, Kidney Structure and Function in Spontaneously Hypertensive Rats with Early Course of Proteinuric Nephropathy.pubmed:27560781
Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4.pubmed:28431936
Chronic restraint stress induces esophageal fibrosis with enhanced oxidative stress in a murine model
NOX5-induced uncoupling of endothelial NO synthase is a causal mechanism and theragnostic target of an age-related hypertension endotypePubmed: 33170835
Biphasic changes in TGF-β1 signaling drive NSAID-induced multi-organ damagePubmed: 32750407