Polyclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1) Homo sapiens (Human) Polyclonal antibody

NOS; nNOS; IHPS1; NC-NOS; N-NOS; bNOS; Neuronal NOS; Constitutive NOS; NOS type I; Nitric Ocide Synthase; Peptidyl-cysteine S-nitrosylase NOS1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA815Hu01-Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1) Packages (Simulation)
  • Polyclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1) Packages (Simulation)
  • PAA815Hu01.jpg Figure. Western Blot; Sample: Recombinant NOS1, Human.
  • Polyclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1) Figure. Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Testis Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against NOS1. It has been selected for its ability to recognize NOS1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric OxidePubMed: 23359814
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • Состояние эндотелиально-сосудистого фактора у беременных с артериальной гипертензиейhandle:123

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