Polyclonal Antibody to Nucleolin (NCL) Mus musculus (Mouse) Polyclonal antibody

C23; Protein C23

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC242Mu01-Recombinant Nucleolin (NCL)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Nucleolin (NCL) Packages (Simulation)
  • Polyclonal Antibody to Nucleolin (NCL) Packages (Simulation)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P;
    Samples: Mouse Cerebellum Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P;
    Samples: Mouse Uterus Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P;
    Samples: Mouse Stomach Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P;
    Samples: Mouse Spinal cord Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P;
    Samples: Mouse Liver Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse NCL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P;
    Sample: Mouse Uterus Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse NCL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) DAB staining on IHC-P;
    Sample: Mouse Cerebellum Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse NCL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nucleolin (NCL) Sample: Mouse Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse NCL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC242Mu01.jpg Figure. Western Blot; Sample: Recombinant NCL, Mouse.
  • Polyclonal Antibody to Nucleolin (NCL) Figure. Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Spleen Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • RNA G-quadruplex as supramolecular carrier for cancer-selective delivery
  • Drug Loading and Functional Efficacy of Cow, Buffalo, and Goat Milk-Derived Exosomes: A Comparative StudyPubmed:35195427
  • Fine Tuning Inclusion in 2 of′-O-Methyl Phosphorothioate Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching ModulationPubmed:34721051

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