Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2) Homo sapiens (Human) Polyclonal antibody

PLA2G6; CaI-PLA2; GVI; INAD1; PNPLA9; Ca2+ Independent PLA2; 85 kDa Calcium-Independent Phospholipase A2; Phospholipase A2,group VI(Cytosolic,Calcium-Independent)

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA411Hu01-Recombinant Phospholipase A2, Calcium Independent (iPLA2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2) Packages (Simulation)
  • Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2) Packages (Simulation)
  • Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2) Figure. DAB staining on IHC-P; Samples: Human Pancreas Tissue.
  • Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2) DAB staining on IHC-P;
    Samples: Human Pancreatic cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human iPLA2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA411Hu01.jpg Figure. Western Blot; Sample: Recombinant iPLA2, Human.
  • Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2) Figure. Western Blot; Sample: Lane1: Human U87-MG Cells; Lane2: Mouse Testis Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against iPLA2. It has been selected for its ability to recognize iPLA2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Anti-cancer effect of snake venom toxin through down regulation of AP-1 mediated PRDX6 expressionPubMed: 26061816
  • Polyunsaturated Fatty Acids and Their Correlations with Parameters of Oxidative/Antioxidant Potential of the Blood and Lipoprotein-Associated Phospholipase A2 in …Pubmed: 30417304
  • Effect of cooling on lung secretory phospholipase A2 activity in vitro, ex vivo and in vivoPubmed: 30604626
  • An investigation of calcium-independent phospholipase A2 (iPLA2) and cytosolic phospholipase A2 (cPLA2) in schizophreniaDoi: 10.1016/j.psychres.2019.01.095
  • Is Admission Lipoprotein-Associated Phospholipase A2 a Novel Predictor of Vasospasm and Outcome in Patients With Aneurysmal Subarachnoid Hemorrhage?Pubmed: 30850840
  • CHANGES IN THE BLOOD FATTY-ACID PROFILE ASSOCIATED WITH OXIDATIVE-ANTIOXIDANT DISTURBANCES IN CORONARY ATHEROSCLEROSIS

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