Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2)
PLA2G6; CaI-PLA2; GVI; INAD1; PNPLA9; Ca2+ Independent PLA2; 85 kDa Calcium-Independent Phospholipase A2; Phospholipase A2,group VI(Cytosolic,Calcium-Independent)
Overview
Properties
- Product No.PAA411Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathway
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA411Mu01-Recombinant Phospholipase A2, Calcium Independent (iPLA2)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 0.22mg/ml
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Specifity
The antibody is a rabbit polyclonal antibody raised against iPLA2. It has been selected for its ability to recognize iPLA2 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Anti-cancer effect of snake venom toxin through down regulation of AP-1 mediated PRDX6 expressionPubMed: 26061816
- Polyunsaturated Fatty Acids and Their Correlations with Parameters of Oxidative/Antioxidant Potential of the Blood and Lipoprotein-Associated Phospholipase A2 in …Pubmed: 30417304
- Effect of cooling on lung secretory phospholipase A2 activity in vitro, ex vivo and in vivoPubmed: 30604626
- An investigation of calcium-independent phospholipase A2 (iPLA2) and cytosolic phospholipase A2 (cPLA2) in schizophreniaDoi: 10.1016/j.psychres.2019.01.095
- Is Admission Lipoprotein-Associated Phospholipase A2 a Novel Predictor of Vasospasm and Outcome in Patients With Aneurysmal Subarachnoid Hemorrhage?Pubmed: 30850840
- CHANGES IN THE BLOOD FATTY-ACID PROFILE ASSOCIATED WITH OXIDATIVE-ANTIOXIDANT DISTURBANCES IN CORONARY ATHEROSCLEROSIS