Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)

CD31; EndoCAM; GPIIA'; PECA1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 95.00 US$ 221.00 US$ 316.00 US$ 790.00 US$ 3,160.00
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Overview

Properties

Product No.PAA363Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryCD & Adhesion molecule Infection immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA363Hu01-Recombinant Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Endometrial cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Prostate Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: THP1 cell
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant PECAM1, Human.
Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Human Lung lysate; Lane3: Human Platelet lysate; Lane4: Rat Spleen lysate; Lane5: 3T3-L1 cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PECAM1. It has been selected for its ability to recognize PECAM1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

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Citations

Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformationsPubmed: 24199192