Polyclonal Antibody to Profilin 1 (PFN1) Homo sapiens (Human) Polyclonal antibody

Epididymis tissue protein Li 184a

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC233Hu01-Recombinant Profilin 1 (PFN1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.43mg/mL
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  • Polyclonal Antibody to Profilin 1 (PFN1) Packages (Simulation)
  • Polyclonal Antibody to Profilin 1 (PFN1) Packages (Simulation)
  • Polyclonal Antibody to Profilin 1 (PFN1) DAB staining on IHC-P; Sample: Porcine Uterus Tissue Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Profilin 1 (PFN1) DAB staining on IHC-P;
    Sample: Porcine Small intestine Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Profilin 1 (PFN1) DAB staining on IHC-P;
    Sample: Porcine Lymph node Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Profilin 1 (PFN1) DAB staining on IHC-P;
    Sample: Porcine Lung Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PFN1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Profilin 1 (PFN1) AF488 staining on IF;
    Sample: A431 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human PFN1 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAC233Hu01.jpg Figure. Western Blot; Sample: Recombinant PFN1, Human.
  • Polyclonal Antibody to Profilin 1 (PFN1) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PFN1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Profilin 1 (PFN1) Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: LO2 cell lysate; Lane3: Rat Liver lysate; Lane4: Mouse Liver lysate; Lane5: Porcine Liver lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human PFN1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PFN1. It has been selected for its ability to recognize PFN1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical ImplementationPubmed:26889680
  • Comparative proteomics analysis of microvesicles in human serum for the evaluation of osteoporosisPubmed: 31081149
  • Evaluation of profilin 1 as a biomarker in myocardial infarctionPubmed: 32767339
  • Reduced perforin release from T cells as a mechanism underlying hypothermia‐mediated neuroprotection
  • ЦИркУлИрУЮщИе актИН-СвЯЗЫваЮщИе БелкИ ПрИ ПроГреССИроваНИИ рака ГортаНИ И ГортаНоГлоткИ
  • The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer
  • Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status34243097

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