Polyclonal Antibody to Prominin 1 (PROM1) Mus musculus (Mouse) Polyclonal antibody

CD133; PROML1; Macular Dystrophy,Retinal 2; Stargardt Disease 4(Autosomal Dominant); Prominin-like protein 1; Antigen AC133

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB516Mu01-Recombinant Prominin 1 (PROM1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.49mg/ml
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  • Polyclonal Antibody to Prominin 1 (PROM1) Packages (Simulation)
  • Polyclonal Antibody to Prominin 1 (PROM1) Packages (Simulation)
  • Polyclonal Antibody to Prominin 1 (PROM1) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse PROM1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prominin 1 (PROM1) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse PROM1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB516Mu01.jpg Western Blot; Sample: Lane1: Mouse Eye lysate; Lane2: Mouse Pancreas lysate; Lane3: U87MG cell lysate Primary Ab: 1µg/ml Rabbit Anti-Mouse PROM1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PROM1. It has been selected for its ability to recognize PROM1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Clinical and biological significance of stem-like CD133+ CXCR4+ cells in esophageal squamous cell carcinomaPubMed: 26092504
  • Hyperglycemia Alters the Protein Levels of Prominin-1 and VEGFA in the Retina of Albino Ratspubmed:29076766
  • Matter, energy and information network of graphene-peptide-based fluorescent sensing system for molecular logic computing, detection and imaging of cancer stem?…Pubmed: 30785136
  • Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and OsteoblastsPubmed: 30838809
  • Prognostic value of stem cell markers in esophageal and esophagogastric junction cancer: a meta-analysisPubmed: 32368307
  • Expression of CD44 and CD133 markers in regional metastases from breast cancer.
  • Antibody CD133 Biofunctionalization of Ammonium Acryloyldimethyltaurate and Vinylpyrrolidone Co-Polymer-Based Coating of the Vascular Implants33321837

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