Polyclonal Antibody to Protease Activated Receptor 2 (PAR2) Mus musculus (Mouse) Polyclonal antibody

F2RL1; F2-RL1; GPR11; PAR2; Coagulation Factor II(thrombin)receptor-Like 1; G-protein coupled receptor 11; Thrombin receptor-like 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA852Mu01-Recombinant Protease Activated Receptor 2 (PAR2)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Protease Activated Receptor 2 (PAR2) Packages (Simulation)
  • Polyclonal Antibody to Protease Activated Receptor 2 (PAR2) Packages (Simulation)
  • Polyclonal Antibody to Protease Activated Receptor 2 (PAR2) Western Blot; Samples: Lane1: NIH/3T3 cell lysate; Lane2: Mouse Kidney lysate; Lane3: Rat Kidney lysate; Lane4: Porcine Kidney lysate;
    Primary Ab: 0.3µg/ml Rabbit Anti-Mouse PAR2 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PAR2. It has been selected for its ability to recognize PAR2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial PneumoniasPubMed: PMC3551240
  • Cathepsin S, a new pruritus biomarker in clinical dandruff/seborrhoeic dermatitis evaluationOnlinelibrary: exd.12357
  • PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosisPubmed:Pmc4079423
  • Proteinase-activated receptor 2 and disease biomarkers in cerebrospinal fluid in cases with autopsy-confirmed prion diseases and other neurodegenerative diseasesPubMed: 25886404
  • Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic RhinosinusitisPubmed:26540312
  • Cathepsin S Alters the Expression of Pro-Inflammatory Cytokines and MMP-9, Partially through Protease—Activated Receptor-2, in Human Corneal Epithelial CellsPubmed: 30423938
  • Whitening effects of cosmetic formulation in the vascular component of skin pigmentationPubmed: 31074159
  • Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡­33924683

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