Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) Homo sapiens (Human) Polyclonal antibody

P58; ERp61; PDIA3; ERp60; GRP57; PI-PLC; GRP58; Glucose Regulated Protein,58kDa; Protein Disulfide Isomerase-Associated 3; Endoplasmic reticulum resident protein 57

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB497Hu01-Recombinant Endoplasmic Reticulum Resident Protein 57 (ERp57)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.45mg/ml
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  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) Packages (Simulation)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) Packages (Simulation)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human PDIA3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) DAB staining on IHC-P;
    Samples: Human Colon Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human PDIA3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) DAB staining on IHC-P;
    Samples: Human Placenta Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human PDIA3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) DAB staining on IHC-P;
    Sample: Human Placenta Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human ERp57 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) DAB staining on IHC-P;
    Sample: Human Colon Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human ERp57 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human ERp57 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB497Hu01.jpg WB Image
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) Western Blot; Sample: Human Lung lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PDIA3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PDIA3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endoplasmic Reticulum Resident Protein 57 (ERp57) Western Blot; Sample: Human A375 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PDIA3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ERp57. It has been selected for its ability to recognize ERp57 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

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