Polyclonal Antibody to R-Spondin 3 (RSPO3) Homo sapiens (Human) Polyclonal antibody

CRISTIN1; PWTSR; THSD2; Thrombospondin,Type I,Domain Containing 2; Protein with TSP type-1 repeat; Roof plate-specific spondin-3

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPM173Hu01-Recombinant R-Spondin 3 (RSPO3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to R-Spondin 3 (RSPO3) Packages (Simulation)
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) Packages (Simulation)
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human RSPO3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) DAB staining on IHC-P;
    Samples: Human Pancreatic cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human RSPO3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human RSPO3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human RSPO3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) DAB staining on IHC-P;
    Samples: Human Bile duct cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human RSPO3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAM173Hu01.jpg Figure. Western Blot; Sample: Recombinant RSPO3, Human.
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) Western Blot; Sample: Porcine Small intestine lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human RSPO3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to R-Spondin 3 (RSPO3) Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Rat Skeletal muscle lysate
    Primary Ab: 5µg/ml Rabbit Anti-Human RSPO3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against RSPO3. It has been selected for its ability to recognize RSPO3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-5µg/mL;
Immunohistochemistry: 5-50µg/mL;
Immunocytochemistry: 5-50µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

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