Polyclonal Antibody to Interferon Gamma (IFNg) Rattus norvegicus (Rat) Polyclonal antibody

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA049Ra01-Recombinant Interferon Gamma (IFNg)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Interferon Gamma (IFNg) Packages (Simulation)
  • Polyclonal Antibody to Interferon Gamma (IFNg) Packages (Simulation)
  • Polyclonal Antibody to Interferon Gamma (IFNg) DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat IFNg Antibod
  • Polyclonal Antibody to Interferon Gamma (IFNg) DAB staining on IHC-P;
    Samples: Rat Kidney Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Rat IFNg Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interferon Gamma (IFNg) DAB staining on IHC-P;
    Samples: Rat Adrenal gland Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Rat IFNg Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interferon Gamma (IFNg) DAB staining on IHC-P;
    Samples: Rat Stomach Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Rat IFNg Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interferon Gamma (IFNg) DAB staining on IHC-P;
    Samples: Rat Heart Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Rat IFNg Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA049Ra01.jpg Figure. Western Blot; Sample: Recombinant IFNg, Rat.
  • Polyclonal Antibody to Interferon Gamma (IFNg) Figure. Western Blot; Sample: Lane1: Mouse Lymphocyte Cells; Lane2: Human Leukocyte Cells.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IFNg. It has been selected for its ability to recognize IFNg in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Bacillus Calmette-Guérin vaccination using a microneedle patchPubMed: 21277407
  • Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease VaccineMedwell: 2010610616
  • Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease VirusMedwell: 2010594600
  • Vaccination with Recombinant Mycobacterium tuberculosis PknD Attenuates Bacterial Dissemination to the Brain in Guinea PigsPubMed: PMC3679071
  • Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in?Escherichia coli?induce potent protective immune responses in guinea pigs, swine and cattlePubMed: PMC3720265
  • Immune Responses to Eimeria tenella Sporozoite Protein as Vaccine to Broiler Against CoccidiosisPjbs: Source
  • Protective effects of neferine on amiodarone-induced pulmonary fibrosis in micePubmed: 23792144
  • Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelPubmed:25476829
  • Protective Effect of Minocycline Against Bacterial Infection-Induced Sickness Behavior in RatsOrg:Source
  • Development of CpG-Oligodeoxynucleotides for Effective Activation of Rabbit TLR9 Mediated Immune ResponsesPubmed:25269083
  • Senescence of tumor cells induced by oxaliplatin increases the efficiency of a lipid A immunotherapy via the recruitment of neutrophilsPubmed:25347345
  • Exogenous carcinoembryonic antigen-related cell adhesion molecule 1 suppresses 2,4,6-trinitrobenzene sulfonic acid-induced ulcerative colitis in micePubmed:25724769
  • Protective effect and mechanism of probucol in the treatment of spinal cord injuryPubMed: 26214485
  • The toxic effect of nickel nanoparticles on oxidative stress and inflammatory markers.Articles:
  • Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbuminPubMed: 25760938
  • Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cellsArticle_14936_6.Html:
  • Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE12 and is crucial for liver transplant tolerancePubMed: 25982596
  • Trypanosoma cruzi infection and benznidazole therapy independently stimulate oxidative status and structural pathological remodeling of the liver tissue in micePubMed: 25912691
  • Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
  • The immunomodulating effect of seminal plasma on T cellsPubMed: 25799173
  • Nanogel‐Incorporated Physical and Chemical Hybrid Gels for Highly Effective Chemo–Protein Combination TherapyDoi: 10.1002
  • Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSFPubMed: 26300317
  • Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4PubMed: 26573719
  • Possible Association of IFN-γ GenePubMed: 26154533
  • Rituximab-induced HMGB1 release is associated with inhibition of STAT3 activity in human diffuse large B-cell lymphomaPubMed: 26315113
  • Construction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunityPubMed: 26607265
  • INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATSjournals:3790
  • Carcinoembryonic antigen related cellular adhesion molecule 1 alleviates dextran sulfate sodium-induced ulcerative colitis in micePubmed:26898127
  • HERPES VIRUS VACCINES AND TREATMENTSy2016:0067330.html
  • Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparatapubmed:27803670
  • Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitispubmed:27553600
  • Reno-protective effect and mechanism study of Huang Lian Jie Du Decoction on lupus nephritis MRL/lpr micepubmed:27825337
  • Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila.pubmed:28366526
  • Evaluation of two strains of Marek's disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus.pubmed:27908832
  • Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.pubmed:28049588
  • Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells.pubmed:27867030
  • Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation09540105.2017.1306494
  • Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathologypubmed:27830033
  • Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthmapubmed:27289030
  • EVALUATION OF HUMORAL AND CELLULAR IMMUNE RESPONSES TO EIMERIA TENELLA OOCYST PROTEIN AS VACCINE TO BROILER363-379.pdf
  • Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury.pubmed:28195073
  • Effects of dietary mixture of garlic (Allium sativum), coriander (Coriandrum sativum) andprobiotics on immune responses and caecal counts in young laying hens.pubmed:27678135
  • Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice.pubmed:28134069
  • Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus.pubmed:28063474
  • Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system.pubmed:28220870
  • Assessment of the efficacy of two novel DNA vaccine formulations against highly pathogenic Porcine Reproductive and Respiratory Syndrome Virus.pubmed:28157199
  • Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period.pubmed:28244338
  • Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in micepubmed:28250847
  • Effect of boron on thymic cytokine expression, hormone secretion, antioxidant functions, cell proliferation, and apoptosis potential via the ERK1/2 signaling pathwaypubmed:29032684
  • Poly (d, l-lactide-co-glycolide) nanoparticle-entrapped vaccine induces a protective immune response against porcine epidemic diarrhea virus infection in pigletspubmed:29102169
  • COMPARATIVE EFFICACY OF TWO HETEROLOGOUS CAPRIPOX VACCINES TO CONTROL LUMPY SKIN DISEASE IN CATTLEISSN 1311-1477
  • The effect of feed supplementation with effective microorganisms(EM) on pro- and anti-inflammatory cytokine concentrations in pigspubmed:28549299
  • Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse modelpubmed:28729731
  • The effects of Spirulina platensis (Arthrospira platensis) and Saccharomyces cerevisiae on the distribution and cytokine production of CD4+ and CD8+ T …S0719-81322017000300185
  • Evaluation of Cell-mediated Immune Response in PBMCs of Calves Vaccinated by Capri Pox Vaccines Using ELISA and Real-time RT-PCRDOI: 10.18869/acadpub.rmm.5.2.3
  • Stress Induced Polarization of Immune-Neuroendocrine Phenotypes in Gallus galluspubmed:28808318
  • Efeito protetor do β-glucano e glutamina em lesões intestinais e imunológicas induzidas por ciratabina (Ara-C) em camundongos10.159:s0100-736x2017000900013
  • Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in micepubmed:28122397
  • An attenuated Shigella mutant lacking the RNA-binding protein Hfq provides cross-protection against Shigella strains of broad serotypepubmed:28727722
  • Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells10.18632/oncotarget.15445
  • A bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccinepubmed:28619143
  • EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-ݜ傠Signaling Pathway in Rats10.1155/2017/3057268
  • Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ.pubmed:28649126
  • EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in Rats10.1155/2017/3057268
  • Associations among interleukin-17, interferon-gamma, and acute coronary syndromeISSN 0970-938X
  • Double-Edged Effect of Hydroxychloroquine on Human Umbilical Cord-Derived Mesenchymal Stem Cells Treating Lupus Nephritis in MRL/lpr MicePubmed:29668284
  • Sonodynamic therapy‐assisted immunotherapy: A novel modality for cancer treatmentPubmed:29575297
  • Identification and preliminary study of immunogens involved in autoimmune prostatitis in human malesPubmed:29947032
  • Immunogenicity and protective efficacy of recombinant fiber-2 protein in protecting SPF chickens against fowl adenovirus 4Pubmed:29395523
  • Silencing of Girdin suppresses the malignant behavior of colorectal carcinoma cellsPubmed:29989653
  • Epstein Barr Virus Infection Affects Function of Cytotoxic T Lymphocytes in Patients with Severe Aplastic AnemiaPubmed:29862282
  • Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
  • Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central ChinaPubmed:29305646
  • Immunomodulatory activity of Lactobacillus plantarum KLDS1. 0318 in cyclophosphamide-treated micePubmed:30026678
  • Involvement of T-Helper 9 Activation in a Mouse Model of Allergic RhinitisPubmed:29982264
  • Proinflammatory Cytokine Production by CD-1 Mice in Response to Equipotential Doses of PG and  …10.1134:S0006297918070088
  • Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitroPubmed:30127867
  • Increased Macroautophagy in Interferon-Gamma-Producing T Cells from Patients with Newly Diagnosed Systemic Lupus ErythematosusPubmed:29941705
  • Construction of Pullorum ghost by co-expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific …10.1016:S2095-3119(17)61696-4
  • Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseasePubmed:29950127
  • Effect of reproductive disorders on productivity and reproductive efficiency of dromedary she-camels in relation to cytokine concentrationPubmed:29423707
  • Immune response characteristics of Capri pox virus vaccines following emergency vaccination of cattle against lumpy skin disease virus65381
  • HUVEC 疫苗联合化疗抗EMT-6 乳腺癌转移实验研究Immune99:Source
  • خصوصیات پاسخ‌های ایمنی القاء شده ناشی از واکسن زنده تخفیف حدت یافته آبله بزی سویه گرگان بر علیه بیماری لمپی اسکین در گاو‎
  • Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in …Pubmed: 30223387
  • PD-1 blockade inhibits lymphocyte apoptosis and restores proliferation and anti-viral immune functions of lymphocyte after CP and NCP BVDV infection in vitroDoi: 10.1016/j.vetmic.2018.10.014
  • NK Cells Suppress CD8+ T Cell Immunity via NKG2D in Severe Aplastic AnemiaDoi: 10.1016/j.cellimm.2018.10.004
  • DNA prime‐protein boost vaccine encoding HLA‐A2,‐A24 and‐DR 1 restricted epitopes of Ca NA 2 against visceral leishmaniasis
  • Intradermal injection of Pythium insidiosum protein antigens for improved diagnosis and treatment of pythiosis in an experimental modelPubmed: 30260397
  • In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6
  • Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic …Pubmed: 30553018
  • Early cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattle
  • Amlexanox attenuates experimental autoimmune encephalomyelitis by inhibiting dendritic cell maturation and reprogramming effector and regulatory T cell …Pubmed: 30823934
  • Construction and Immunogenicity of Novel Chimeric Virus-Like Particles Bearing Antigens of Infectious Bronchitis Virus and Newcastle Disease VirusPubmed: 30871190
  • Hyperandrogenism and insulin resistance induce gravid uterine defects in association with mitochondrial dysfunction and aberrant ROS productionPubmed: 30860876
  • Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD MicePubmed: 30980800
  • Local probiotic Lactobacillus crispatus and Lactobacillus delbrueckii exhibit strong antifungal effects against vulvovaginal candidiasis in a rat model
  • Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal OriginPubmed: 31183654
  • Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in ChickensPubmed: 31137731
  • Magnetic-responsive and targeted cancer nanotheranostics by PA/MR bimodal imaging-guided photothermally triggered immunotherapy
  • Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus
  • Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis
  • Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in ratPubmed: 31255668

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