• KO-Validated
Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Rattus norvegicus (Rat) Polyclonal antibody

IPO-B; MNSOD; Mn-SOD

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB083Ra01-Recombinant Superoxide Dismutase 2, Mitochondrial (SOD2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Packages (Simulation)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Packages (Simulation)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P; Samples: Rat Uterus Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Samples: Rat Testis Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Samples: Rat Kidney Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Samples: Rat Skeletal muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Samples: Rat Cardiac Muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Samples: Rat Liver Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Sample: Rat Uterus Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat SOD2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Sample: Rat Testis Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat SOD2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) DAB staining on IHC-P;
    Sample: Rat Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat SOD2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) FITC staining on IF;Sample: MCF7 cellPrimary Ab: 20µg/ml Rabbit Anti-Rat SOD2 AntibodySecond Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb11)
  • PAB083Ra01.jpg Figure. Western Blot; Sample: Recombinant SOD2, Rat.
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: SOD2 knockout Hela cell lysate;
    Predicted MW: 25kDa
    Observed MW: 22kDa
    Primary Ab: 1µg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Western Blot; Sample: Rat Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Western Blot; Sample: Rat Cerebrum lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Western Blot; Sample: Rat Lung lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat SOD2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SOD2. It has been selected for its ability to recognize SOD2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Antioxidant profile of salivary glands in high fat diet-induced insulin resistance ratsPubmed: 24106991
  • Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closurePubmed:24443098
  • Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal CancerPubmed:25550558
  • Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetesPubMed: 26143097
  • Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
  • DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts.Pubmed:26806551
  • Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and …Pubmed:29892894
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
  • A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 SignalingPubmed: 32135248
  • Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease DevelopmentPubmed: 32751983
  • Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­34064937
  • Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients34832849
  • α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat dietPubmed:35569738

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