Polyclonal Antibody to Tumor Protein p53 (P53) Rattus norvegicus (Rat) Polyclonal antibody

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA928Ra01-Recombinant Tumor Protein p53 (P53)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (P53) FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20µg/mL Rabbit Anti-Rat TP53 Antibody Second Ab: 1.5µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Tumor Protein p53 (P53) FITC staining on IF;
    Samples: Human Hela cell;
    Primary Ab: 40µg/mL Rabbit Anti-Rat TP53 Antibody
    Second Ab: 1.5µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAA928Ra01.jpg Western Blot; Sample: 293T cell lysate Primary Ab: 0.5µg/ml Rabbit Anti-Rat P53 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: HaCaT cell lysate
    Primary Ab: 0.5µg/ml Rabbit Anti-Rat P53 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunofluorescence:5-40µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Analysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Therapeutic role of curcumin in oxidative DNA damage caused by formaldehydePubMed: 25761397
  • Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing micePubMed: 26344777
  • Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytesPubmed: 31331641
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …

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