Polyclonal Antibody to Ribophorin I (RPN1) Homo sapiens (Human) Polyclonal antibody

RPNI; OST1; RBPH1; Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1; Dolichyl-diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC769Hu01-Recombinant Ribophorin I (RPN1)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Ribophorin I (RPN1) Packages (Simulation)
  • Polyclonal Antibody to Ribophorin I (RPN1) Packages (Simulation)
  • Polyclonal Antibody to Ribophorin I (RPN1) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human RPN1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ribophorin I (RPN1) DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human RPN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ribophorin I (RPN1) DAB staining on IHC-P;
    Samples: Human Lung cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human RPN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ribophorin I (RPN1) DAB staining on IHC-P;
    Samples: Human Pancreas Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human RPN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ribophorin I (RPN1) DAB staining on IHC-P;
    Sample: Human Placenta Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human RPN1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ribophorin I (RPN1) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human RPN1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC769Hu01.jpg Figure. Western Blot; Sample: Recombinant RPN1, Human.
  • Polyclonal Antibody to Ribophorin I (RPN1) Western Blot; Sample: Porcine Pancreas lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human RPN1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ribophorin I (RPN1) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human RPN1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ribophorin I (RPN1) Western Blot; Samples: Lane1: Porcine Pancreas lysate; Lane2: Porcine Stomach lysate; Lane3: Hela cell lysate; Lane4: BXPC3 cell lysate;
    Primary Ab: 0.2µg/ml Rabbit Anti-Human RPN1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against RPN1. It has been selected for its ability to recognize RPN1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

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