KO-Validated
Polyclonal Antibody to S100 Calcium Binding Protein A2 (S100A2)

S100-A2; CAN19; S100L

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAC009Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryTumor immunity Infection immunity Reproductive science

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC009Hu01-Recombinant S100 Calcium Binding Protein A2 (S100A2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

Used in DAB staining on fromalin fixed paraffin-embedded kidney tissue

Figure. Western Blot; Sample: Recombinant S100A2, Human.
Knockout Varification:
Lane 1: Wild-type BXPC3 cell lysate;
Lane 2: S100A2 knockout BXPC3 cell lysate;
Predicted MW: 11kd
Observed MW: 10kd
Primary Ab: 3µg/ml Rabbit Anti-Human S100A2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Porcine Esophagus lysate; Lane2: HaCaT cell lysate;
Primary Ab: 0.15µg/ml Rabbit Anti-Human S100A2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against S100A2. It has been selected for its ability to recognize S100A2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Diagnostic significance of S100A2 and S100A6 levels in sera of patients with non-small cell lung cancerPubMed: 26361956
S100A2: A potential biomarker to differentiate malignant from tuberculous pleural effusionS100A2: A potential biomarker to differentiate malignant from …

KO-Validated Polyclonal Antibody to S100 Calcium Binding Protein A2 (S100A2)