Monoclonal Antibody to S100 Calcium Binding Protein (S100)
S100-A1; S100A1; S100-Alpha; S-100 Protein; S-100 protein subunit alpha; S-100 protein alpha chain
Overview
Properties
- Product No.MAA012Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryTumor immunity
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 Kappa, Clone Number C8
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA012Hu01-Recombinant S100 Calcium Binding Protein (S100)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against S100. It has been selected for its ability to recognize S100 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Sus scrofa; Porcine (Pig)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Vasoactive intestinal peptide protects against ischemic brain damage induced by focal cerebral ischemia in ratsScienceDirect: S0006899311008663
- A New Technique for Collection of Cerebrospinal Fluid in Rat PupsPubMed: 26056488
- Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methioninepubmed:27501393
- Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.pubmed:28238669
- Levels of neurotropic autoantibodies in patients with schizophreniaarticle:n
- Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayPubmed:29770336
- Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanismsPubmed: 30421246
- ВОЗМОЖНОСТЬ ПРОГНОЗИРОВАНИЯ ОСТРОЙ НЕСПЕЦИФИЧЕСКОЙ БОЛИ В НИЖНЕЙ ЧАСТИ СПИНЫ С ИСПОЛЬЗОВАНИЕМ СЫВОРОТОЧНЫХ …
- ВЛИЯНИЕ ХРОНИЧЕСКОГО ВВЕДЕНИЯ НЕЙРОЛЕПТИКОВ НА УРОВНИ НЕЙРОСПЕЦИФИЧЕСКИХ АУТОАНТИТЕЛ В КРОВИ У КРЫС