Monoclonal Antibody to S100 Calcium Binding Protein (S100) Homo sapiens (Human) Monoclonal antibody

S100-A1; S100A1; S100-Alpha; S-100 Protein; S-100 protein subunit alpha; S-100 protein alpha chain

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number n/a
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to S100 Calcium Binding Protein (S100) Packages (Simulation)
  • Monoclonal Antibody to S100 Calcium Binding Protein (S100) Packages (Simulation)
  • Monoclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 30μg/ml Mouse Anti-Human S100 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • MAA012Hu22.jpg Western Blot; Sample: Human HT1080 cell lysate; Primary Ab: 2µg/ml Mouse Anti-Human S100 Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against S100. It has been selected for its ability to recognize S100 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Citations

  • Vasoactive intestinal peptide protects against ischemic brain damage induced by focal cerebral ischemia in ratsScienceDirect: S0006899311008663
  • A New Technique for Collection of Cerebrospinal Fluid in Rat PupsPubMed: 26056488
  • Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methioninepubmed:27501393
  • Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.pubmed:28238669
  • Levels of neurotropic autoantibodies in patients with schizophreniaarticle:n
  • Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayPubmed:29770336
  • Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanismsPubmed: 30421246

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