Polyclonal Antibody to S100 Calcium Binding Protein (S100) Mus musculus (Mouse) Polyclonal antibody

S100-A1; S100A1; S100-Alpha; S-100 Protein; S-100 protein subunit alpha; S-100 protein alpha chain

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA012Mu01-Recombinant S100 Calcium Binding Protein (S100)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.33mg/ml
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  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) Packages (Simulation)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) Packages (Simulation)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P; Sample: Mouse Cerebrum Tissue Primary Ab: 10µg/ml Rabbit Anti-Mouse S100 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P;
    Samples: Mouse Lymph node Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse S100 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P;
    Samples: Mouse Heart Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse S100 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P;
    Samples: Mouse Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse S100 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P;
    Samples: Mouse Stomach Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse S100 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P;
    Sample: Mouse Cardiac Muscle Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse S100 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse S100 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to S100 Calcium Binding Protein (S100) AF488 staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Mouse S100 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA012Mu01.jpg Western Blot; Sample: Lane1: Bovine Heart lysate; Lane2: Rat Heart lysate; Lane3: Mouse Heart lysate Primary Ab: 0.5µg/ml Rabbit Anti-Mouse S100 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against S100. It has been selected for its ability to recognize S100 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat), Bos taurus; Bovine (Cattle)

Giveaways

Citations

  • Vasoactive intestinal peptide protects against ischemic brain damage induced by focal cerebral ischemia in ratsScienceDirect: S0006899311008663
  • A New Technique for Collection of Cerebrospinal Fluid in Rat PupsPubMed: 26056488
  • Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methioninepubmed:27501393
  • Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.pubmed:28238669
  • Levels of neurotropic autoantibodies in patients with schizophreniaarticle:n
  • Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayPubmed:29770336
  • Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanismsPubmed: 30421246
  • ВОЗМОЖНОСТЬ ПРОГНОЗИРОВАНИЯ ОСТРОЙ НЕСПЕЦИФИЧЕСКОЙ БОЛИ В НИЖНЕЙ ЧАСТИ СПИНЫ С ИСПОЛЬЗОВАНИЕМ СЫВОРОТОЧНЫХ …
  • ВЛИЯНИЕ ХРОНИЧЕСКОГО ВВЕДЕНИЯ НЕЙРОЛЕПТИКОВ НА УРОВНИ НЕЙРОСПЕЦИФИЧЕСКИХ АУТОАНТИТЕЛ В КРОВИ У КРЫС

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