Polyclonal Antibody to SHC-Transforming Protein 1 (SHC1)

SHC; SHCA; p52SHC; p66; p66SHC; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAE671Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE671Hu01-Recombinant SHC-Transforming Protein 1 (SHC1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.47mg/ml

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Packages (Simulation)
Packages (Simulation)

Used in DAB staining on fromalin fixed paraffin-embedded stomach tissue

Figure. Western Blot; Sample: Recombinant SHC1, Human.
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human SHC1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human SHC1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SHC1. It has been selected for its ability to recognize SHC1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:230-940
Immunohistochemistry: 5-20µg/mL;1:23-94
Immunocytochemistry: 5-20µg/mL;1:23-94
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment.pubmed:28063625