Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) Homo sapiens (Human) Polyclonal antibody

FRP-4; FRPHE; Frizzled protein, human endometrium

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPF878Hu01-Recombinant Secreted Frizzled Related Protein 4 (SFRP4)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) Packages (Simulation)
  • Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) Packages (Simulation)
  • Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) IHC Images
  • PAF878Hu01.jpg Figure. Western Blot; Sample: Recombinant SFRP4, Human.
  • Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human SFRP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SFRP4. It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:250-1000
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerancePubMed: 26041556
  • Secreted Frizzled-Related Protein 4 (SFRP4) is Elevated in Patients with Diabetes MellitusPubmed:26882051
  • Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesitypubmed:27768254
  • Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican AmericansPubmed:29427376
  • Novel marker for gestational diabetespatent:US20180059122A1
  • A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profilePubmed: 30375290
  • SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinomaPubmed: 30906634
  • Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic …Pubmed: 31011777
  • First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitusPubmed: 33206702
  • SFRP4 drives invasion in gastric cancer and is an early predictor of recurrence33277667
  • Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop …

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