KO-Validated
Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7)

CDw328; CD328; p75; AIRM1; D-Siglec; QA79; SIGLEC-7; P75/AIRM1; Adhesion inhibitory receptor molecule 1; QA79 membrane protein

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 100.00 US$ 232.00 US$ 332.00 US$ 830.00 US$ 3,320.00
Quantity

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Overview

Properties

Product No.PAB789Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryCD & Adhesion molecule Tumor immunity Infection immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB789Hu01-Recombinant Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human SIGLEC7 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant SIGLEC7, Human.
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human SIGLEC7 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: SIGLEC7 knockout A549 cell lysate;
Predicted MW: 51,41,16kd
Observed MW: 41kd
Primary Ab: 3µg/ml Rabbit Anti-Human SIGLEC7 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SIGLEC7. It has been selected for its ability to recognize SIGLEC7 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-3µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.