Polyclonal Antibody to Sirtuin 3 (SIRT3) Rattus norvegicus (Rat) Polyclonal antibody

SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPE913Ra01-Recombinant Sirtuin 3 (SIRT3)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Sirtuin 3 (SIRT3) Packages (Simulation)
  • Polyclonal Antibody to Sirtuin 3 (SIRT3) Packages (Simulation)
  • Polyclonal Antibody to Sirtuin 3 (SIRT3) DAB staining on IHC-P; Sample: Rat Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-Rat SIRT3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Sirtuin 3 (SIRT3) DAB staining on IHC-P;
    Sample: Rat Colon Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Rat SIRT3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Sirtuin 3 (SIRT3) DAB staining on IHC-P;
    Sample: Rat Cerebrum Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Rat SIRT3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Sirtuin 3 (SIRT3) DAB staining on IHC-P;
    Sample: Rat Lymph node Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Rat SIRT3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAE913Ra01.jpg Figure. Western Blot; Sample: Recombinant SIRT3, Rat.
  • Polyclonal Antibody to Sirtuin 3 (SIRT3) Western Blot; Sample: Lane1: Rat Skeletal muscle lysate; Lane2: 293T cell lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Rat SIRT3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SIRT3. It has been selected for its ability to recognize SIRT3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • SirT3 regulates diabetogenic effects caused by arsenic: An implication for mitochondrial complex II modificationPubmed: 30385301
  • Effects of perinatal factors on sirtuin 3, 8-hydroxy-2′-deoxyguanosine, brain-derived neurotrophic factor and serotonin in cord blood and early breast milk: an …Pubmed: 32552911
  • The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡­33396247

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