Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) Homo sapiens (Human) Polyclonal antibody

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPE698Hu01-Recombinant Sphingosine Kinase 1 (SPHK1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) Packages (Simulation)
  • Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) Packages (Simulation)
  • Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) DAB staining on IHC-P; Sample: Porcine Kidney Tissue Primary Ab: 10µg/ml Rabbit Anti-Human SPHK1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human SPHK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAE698Hu01.jpg Figure. Western Blot; Sample: Recombinant SPHK1, Human.
  • Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) Figure. Western Blot; Lane1: Human Raji Tissue; Lane2: Mouse Lung Tissue.
  • Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1) Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human SPHK1 Polyclonal Antibody (Catalog # PAE698Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SPHK1. It has been selected for its ability to recognize SPHK1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Sus scrofa; Porcine (Pig)

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