Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 
CSC-21K; TIMP Metallopeptidase Inhibitor 2
Overview
Properties
- Product No.PAA128Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryTumor immunity
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA128Hu01-Recombinant Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human TIMP2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Mammary gland Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human TIMP2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human TIMP2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human TIMP2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human TIMP2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human TIMP2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant TIMP2, Human.
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against TIMP2. It has been selected for its ability to recognize TIMP2 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Oral Administration of 3,3′-Diindolylmethane Inhibits Lung Metastasis of 4T1 Murine Mammary Carcinoma Cells in BALB/c MiceNutrition&Metabolism: source
- Oral administration of benzyl-isothiocyanate inhibits solid tumor growth and lung metastasis of 4T1 murine mammary carcinoma cells in BALB/c miceSpringerLink: f3w831267267p277
- Bone morphogenetic protein-2: a potential regulator in scleral remodelingPubMed: PMC2605409
- Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental strokePubMed: 21679435
- A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
- Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative coursePubmed: 23585206
- Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
- Impact of 6% hydroxyethyl starch 130/0.42 and 5% gelatin on renal function in a pediatric animal modelPubmed:24916275
- The Complementary Effects of Atorvastatin and Exercise Treatment on the Composition and Stability of the Atherosclerotic Plaques in ApoE Knockout MicePubmed:25264981
- Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model Pubmed:25266150
- Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the HorseJournals: Mi
- Plasma inflammatory biomarkers for Huntington’s disease patients and mouse modelPubMed: 25266150
- The skin remodeling in type 1 diabetes and insulin resistance animal modelsPubMed: 26047379
- The skin remodeling in type 1 diabetes and insulin resistance animal models.PubMed: 26047379
- Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferolpmc:PMC4848383
- From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
- Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction.pubmed:27938355
- Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis 10.108:09712119.2017.1335208
- The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cellspubmed:29224019
- From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
- Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis10.1080:09712119.2017.1335208
- Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma
- Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular …Pubmed: 31464166
- Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosisPubmed: 31980722
- MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic33488296
- Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer34650982