Polyclonal Antibody to Topoisomerase II (TOP2) Homo sapiens (Human) Polyclonal antibody

DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA792Hu01-Recombinant Topoisomerase II (TOP2)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Topoisomerase II (TOP2) Packages (Simulation)
  • Polyclonal Antibody to Topoisomerase II (TOP2) Packages (Simulation)
  • Polyclonal Antibody to Topoisomerase II (TOP2) DAB staining on IHC-P; Samples: Human Tonsil Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human TOP2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Topoisomerase II (TOP2) DAB staining on IHC-P;
    Samples: Human Endometrial cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human TOP2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA792Hu01.jpg Figure. Western Blot; Sample: Recombinant TOP2, Human.
  • Polyclonal Antibody to Topoisomerase II (TOP2) Western Blot; Sample: Human Serum;
    Primary Ab: 1µg/ml Rabbit Anti-Human TOP2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against TOP2. It has been selected for its ability to recognize TOP2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase IIpubmed:27852319
  • Synthesis of pharmacologically important naphthoquinones and anticancer activity of 2-benzyllawsone through DNA topoisomerase-II inhibition.pubmed:28094224
  • Antiproliferative activity of diarylnaphthylpyrrolidine derivative via dual target inhibitionPubmed: 31884407
  • 2-{5-[(Z,2Z)-2-Chloro-3-(4-nitrophenyl)-2-propenylidene]-4-oxo-2-thioxothiazolidin-3-yl}-3-methylbutanoic Acid as a Potential Anti-Breast Cancer MoleculePubmed:35456915

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