Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha
Overview
Properties
- Product No.PAA134Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryCytokineTumor immunityInfection immunity
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA134Hu01-Recombinant Tumor Necrosis Factor Beta (TNFb)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 0.5mg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against TNFb. It has been selected for its ability to recognize TNFb in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling processPubMed: 22984252
- Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantationPubmed:26847569
- Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected CytokinesIJST-2328-3548-04-803
- Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
- Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Pubmed: 32680553
- Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
- Elevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration33441130