Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF) Homo sapiens (Human) Monoclonal antibody

NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number 9#
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB166Hu01-Recombinant VGF Nerve Growth Factor Inducible (VGF)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF) Packages (Simulation)
  • Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF) Packages (Simulation)
  • Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF) DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 30µg/ml Mouse Anti-Human VGF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF) DAB staining on IHC-P;
    Sample: Human Glioma Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Human VGF Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB166Hu22.jpg Figure. Western Blot; Sample: Recombinant VGF, Human.
  • Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF) Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Placenta lysate; Lane3: U87MG cell lysate
    Primary Ab: 3μg/ml Mouse Anti-Human VGF Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against VGF. It has been selected for its ability to recognize VGF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Proteomic analysis of cerebrospinal fluid from obese women with idiopathic intracranial hypertension: a new approach for identifying new candidates in the pathogenesis of obesityPubMed: 22296024
  • Low VGF is associated with executive dysfunction in patients with major depressive disorderPubmed:35738161

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