Polyclonal Antibody to Vasoactive Intestinal Peptide (VIP)
PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
Overview
Properties
- Product No.PAA380Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyGastroenterologyHormone metabolism
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA380Mu01-Recombinant Vasoactive Intestinal Peptide (VIP)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 0.25mg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against VIP. It has been selected for its ability to recognize VIP in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Rattus norvegicus (Rat)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese populationWiley: source
- Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathyPubmed: 23800435
- CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic MigraineOnlinelibrary: head.12372
- Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptideNCBI: PMC4000510
- Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migrainePubmed:24847167
- Clinical study of electro-acupuncture treatment with different intensities for functional constipation patientsPubmed:25318892
- Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 TransportersPubmed:25647228
- Aqueous Fraction ofPubMed: 25647228
- Oral curcumin has anti-arthritic efficacy through somatostatin generationPubMed: 25836921
- Effects of sphincter of Oddi motility on the formation of cholesterol gallstonesPubmed:27350732
- Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitisPubmed:27156748
- Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patientsPubmed:27250233
- No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migrainepubmed:27634731
- Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut.pubmed:28054652
- [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice10.1186/s12906-017-1903-0
- Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation10.1007/s13765-017-0327-3
- Salivary neuropeptides, stress, and periodontitisPubmed:28914594
- Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile AcidsPubmed:29853946
- Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patientsPubmed: 30365640
- Serum Levels of Neuropeptides in Cows with Left Abomasal DisplacementPubmed: 30562932
- Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric UlcerPubmed: 30479041
- Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practicePubmed: 32951099
- Disrupted local innervation results in less VIP expression in CF mice tissuesPubmed: 32600901
- Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
- Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome
- VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD)34034984
- Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study34698942
- A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experiencePubmed:35644576