Polyclonal Antibody to Vinculin (VCL) Homo sapiens (Human) Polyclonal antibody

MVCL; Metavinculin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB839Hu01-Recombinant Vinculin (VCL)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Vinculin (VCL) Packages (Simulation)
  • Polyclonal Antibody to Vinculin (VCL) Packages (Simulation)
  • Polyclonal Antibody to Vinculin (VCL) Used in DAB staining on fromalin fixed paraffin-embedded stomach tissue
  • PAB839Hu01.jpg Figure. Western Blot; Sample: Recombinant VCL, Human.
  • Polyclonal Antibody to Vinculin (VCL) Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Cerebrum lysate; Lane3: Porcine Heart lysate; Lane4: Bovine Liver lysate; Lane5: Bovine Heart lysate
    Primary Ab: 5µg/mL Rabbit Anti-Human VCL Ab
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-50µg/mL;
Immunocytochemistry: 5-50µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

Citations

  • S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failurejournals:1384523
  • PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signalsPubmed:35044463

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