Polyclonal Antibody to Vinculin (VCL) Homo sapiens (Human) Polyclonal antibody

MVCL; Metavinculin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB839Hu01-Recombinant Vinculin (VCL)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Vinculin (VCL) Packages (Simulation)
  • Polyclonal Antibody to Vinculin (VCL) Packages (Simulation)
  • Polyclonal Antibody to Vinculin (VCL) Used in DAB staining on fromalin fixed paraffin-embedded stomach tissue
  • PAB839Hu01.jpg Figure. Western Blot; Sample: Recombinant VCL, Human.
  • Polyclonal Antibody to Vinculin (VCL) Figure. Western Blot; Sample. Lane1: Mouse Heart Tissue; Lane2: Human Lung Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Citations

  • S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failurejournals:1384523

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