Biotin-Linked Polyclonal Antibody to E-cadherin Sus scrofa; Porcine (Pig) Labelled antibody

CDHE; CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule

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Overview
Properties
  • SourceAntibody labeling
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelBiotin
  • Original Antibody n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Biotin-Linked Polyclonal Antibody to E-cadherin Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to E-cadherin Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to E-cadherin Western Blot; Sample:Porcine Liver lysate
    Primary Ab: 0.2µg/ml Biotin-Linked Polyclonal Antibody to E-cadherin
    Second Ab: 0.2µg/ml HRP-Labeled Streptavidin (Catalog: IS089)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against E-cadherin. It has been selected for its ability to recognize E-cadherin in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Novel method for proliferation of oral keratinocyte stem cellsPubmed: 24329011
  • Modulation of the Dual‐Faced Effects of miR‐141 with Chitosan/miR‐141 Nanoplexes in Breast Cancer CellsPubmed: 31389101
  • BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherinPubmed: 31957537
  • Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy …Pubmed: 32342301
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …

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