Biotin-Linked Polyclonal Antibody to Cytochrome P450 1A2 (CYP1A2) 
CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2
Overview
Properties
- Product No.LAD294Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategoryMetabolic pathway
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Sample: Mouse Liver lysate
Primary Ab: 0.2µg/ml Biotin-Linked Polyclonal Antibody to Cytochrome P450 1A2 (CYP1A2)
Second Ab: 0.2µg/ml HRP-Labeled Streptavidin (Catalog: IS089)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against CYP1A2. It has been selected for its ability to recognize CYP1A2 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytesPubmed:25471622
- Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4PubMed: 25733458
- Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar ratsPubmed:27133590
- Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats.pubmed:27133590
- Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stressb0407ebde042c4f61b728bc11a586e6c
- Liver lobe and strain differences in the activity of murine cytochrome p450 enzymesPubmed:29879457
- Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus