Biotin-Linked Polyclonal Antibody to Cytochrome P450 2E1 (CYP2E1)
CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1
Overview
Properties
- Product No.LAA988Mu71
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategorySignal transductionMetabolic pathway
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against CYP2E1. It has been selected for its ability to recognize CYP2E1 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in ratsPubMed: PMC3605171
- Mechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in RatsPubMed: PMC3749608
- CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in ratPubMed: PMC3519517
- Hepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. Ajbpr:Source
- Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in ratsPubmed:24701154
- Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivopublication:307092820
- Haloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats.59f7c85aaca272607e2d8d7a
- Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixturesPubmed: 31639605
- Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic BeveragesPubmed: 31935882
- Ellagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress34573017