Biotin-Linked Polyclonal Antibody to Fatty Acid Binding Protein 4 (FABP4) Sus scrofa; Porcine (Pig) Labelled antibody

A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein

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Overview
Properties
  • SourceAntibody labeling
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelBiotin
  • Original Antibody n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Biotin-Linked Polyclonal Antibody to Fatty Acid Binding Protein 4 (FABP4) Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to Fatty Acid Binding Protein 4 (FABP4) Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to Fatty Acid Binding Protein 4 (FABP4) Western Blot; Sample:Porcine Heart lysate
    Primary Ab: 0.2µg/ml Biotin-Linked Polyclonal Antibody to Fatty Acid Binding Protein 4 (FABP4)
    Second Ab: 0.2µg/ml HRP-Labeled Streptavidin (Catalog: IS089)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against FABP4. It has been selected for its ability to recognize FABP4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASHPubMed: 23091808
  • Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adultsPubMed: 26134622
  • Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic micePubMed: 25867423
  • Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytesPubMed: 25952883
  • Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsiaPubmed:26992681
  • Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies.pubmed:28161274
  • Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …Pubmed:29849871
  • Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humansPubmed:29335416
  • 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4Pubmed: 31720079
  • Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074
  • The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart FailurePubmed:35455943
  • FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritisPubmed:35729106

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