Biotin-Linked Polyclonal Antibody to Fibulin 3 (FBLN3) 
EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; Fibrillin-Like; Extracellular protein S1-5
Overview
Properties
- Product No.LAF422Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathway
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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WB Image
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against FBLN3. It has been selected for its ability to recognize FBLN3 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)Giveaways
Increment services
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
Citations
- Fibulin-3 as a Blood and Effusion Biomarker for Pleural MesotheliomaPubMed: PMC3761217
- YKL-40 and mesothelin in the blood of patients with malignant mesothelioma, lung cancer and asbestosis.Pubmed: 24324091
- Role of fibulin-3 in lung cancer: In vivo and in vitro analysesSpandidos-publications: Source
- Discovery of new biomarkers for malignant mesotheliomaSpringer:Source
- DIAGNOSIS OF CELL PROLIFERATIVE DISEASESFreepatentsonline:Source
- Comparison of fibulin-3 and mesothelin as markers in malignant mesotheliomaPubmed:Pmc4174124
- Value of plasma and pleural effusion fibulin-3 levels in the diagnosis of malignant pleural mesothelioma effusionsPubmed:23050525
- Fibulin-3 as a biomarker of response to treatment in malignant mesotheliomaPubMed: 26401134
- Fibulin-3 levels in malignant pleural mesothelioma are associated with prognosis but not diagnosisPubMed: 26263483
- Plasma Biomarker Enrichment of Clinical Prognostic Indices in Malignant Pleural Mesothelioma.Pubmed:26903362
- Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational study pubmed:27884852
- HMGB1 and its hyperacetylated isoform are sensitive and specific serum biomarkers to detect asbestos exposure and to identify mesothelioma patientspubmed:26733616
- Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational …e013324.abstract
- Comparison of the Diagnostic Performance of Fibulin-3 and Mesothelin in Patients with Pleural Effusions from Malignant Mesothelioma.pubmed:28314308
- A Novel Panel of Serum Biomarkers for MPM Diagnosis.pubmed:28348450
- Association of fibulin-3 concentrations with the presence and severity of knee osteoarthritis: A cross-sectional studypubmed:29195846
- Plasma Fibulin-3 as a Potential Biomarker for Patients with Asbestos-Related Diseases in the Han Populationpubmed:29200597
- Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranesPubmed:29749501
- The matrix protein Fibulin-3 promotes KISS1R induced triple negative breast cancer cell invasionPubmed:30046386
- Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with …68362.pdf
- Prediction of pneumoconiosis by serum and urinary biomarkers in workers exposed to asbestos-contaminated mineralsPubmed: 30946771